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Product Info Summary
| SKU: | A06762 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Hormone-sensitive lipase Lipe Antibody
View all Hormone-sensitive Lipase/HSL Antibodies
SKU/Catalog Number
A06762
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hormone-sensitive lipase Lipe Antibody (Catalog # A06762). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Lipe antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Hormone-sensitive lipase Lipe Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06762)
Host
Rabbit
Contents
Lipe Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Lipe antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Lipe. The immunogen is located within amino acids 920 - 970 of Lipe.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of Lipe are known to exist.
Reactive Species
A06762 is reactive to LIPE in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
116598 MW
Background of Hormone-sensitive Lipase/HSL
Although initially described as an adipocyte-specific triacylglycerol lipase, Lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of Lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that Lipe plays a major role in adipocyte proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06762 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Lipe antibody can be used for detection of Lipe by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Lipe in human lymph node tissue lysate with Lipe antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of lipe in human lymph node tissue with lipe antibody at 2.5 μg/mL.
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Immunofluorescence of Lipe in Human Lymph Node cells with Lipe antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For LIPE (Source: Uniprot.org, NCBI)
Gene Name
LIPE
Full Name
Hormone-sensitive lipase
Weight
116598 MW
Superfamily
GDXG' lipolytic enzyme family
Alternative Names
HSL, LHS, Hormone-sensitive lipase, HSL LIPE AOMS4, FPLD6, HSL, LHS, REH lipase E, hormone sensitive type hormone-sensitive lipase|lipase, hormone-sensitive|monoacylglycerol lipase LIPE|retinyl ester hydrolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIPE, check out the LIPE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIPE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hormone-sensitive lipase Lipe Antibody (A06762)
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