Product Info Summary
SKU: | M00389-4 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HNF-4-alpha Rabbit Monoclonal Antibody
View all HNF-4 alpha/NR2A1 Antibodies
SKU/Catalog Number
M00389-4
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-HNF-4-alpha Rabbit Monoclonal Antibody catalog # M00389-4. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HNF-4-alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00389-4)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
28H20
Isotype
IgG
Immunogen
A synthesized peptide derived from human HNF-4-alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00389-4 is reactive to HNF4A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
53 kDa
Calculated molecular weight
52785 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00389-4 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HNF-4-alpha expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For HNF4A (Source: Uniprot.org, NCBI)
Gene Name
HNF4A
Full Name
Hepatocyte nuclear factor 4-alpha
Weight
52785 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Hepatocyte nuclear factor 4-alpha;HNF-4-alpha;Nuclear receptor subfamily 2 group A member 1;Transcription factor 14;TCF-14;Transcription factor HNF-4;HNF4A;HNF4, NR2A1, TCF14; HNF4A FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14 hepatocyte nuclear factor 4 alpha hepatocyte nuclear factor 4-alpha|HNF4alpha10/11/12|TCF-14|hepatic nuclear factor 4 alpha|nuclear receptor subfamily 2 group A member 1|transcription factor 14|transcription factor HNF-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNF4A, check out the HNF4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNF4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HNF-4-alpha Rabbit Monoclonal Antibody (M00389-4)
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