Product Info Summary
SKU: | A06717 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Histone H1 HIST1H1B Antibody
SKU/Catalog Number
A06717
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Histone H1 HIST1H1B Antibody catalog # A06717. Tested in EILSA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H1 HIST1H1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06717)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of T17.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06717 is reactive to HIST1H1B in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
22580 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06717 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Click image to see more details
Western blot analysis of various cells using Histone H1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Protein Target Info & Infographic
Gene/Protein Information For HIST1H1B (Source: Uniprot.org, NCBI)
Gene Name
HIST1H1B
Full Name
Histone H1.5
Weight
22580 MW
Superfamily
histone H1/H5 family
Alternative Names
Histone H1.5;Histone H1a;Histone H1b;Histone H1s-3;HIST1H1B;H1F5; H1-5 H1, H1.5, H1B, H1F5, H1s-3, HIST1H1B H1.5 linker histone, cluster member histone H1.5|H1 histone family, member 5|histone 1, H1b|histone H1a|histone H1b|histone H1s-3|histone cluster 1 H1 family member b|histone cluster 1, H1b
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H1B, check out the HIST1H1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H1 HIST1H1B Antibody (A06717)
Hello CJ!
A06717 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Role of endoplasmic reticulum stress in apoptosis of differentiated mouse podocytes induced by high glucose
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Customer Q&As
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3 Customer Q&As for Anti-Histone H1 HIST1H1B Antibody
Question
Is this A06717 anti-Histone H1 antibody reactive to the isotypes of HIST1H1B?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
The immunogen of A06717 anti-Histone H1 antibody is Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of T17. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-Histone H1 antibody A06717 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
The anti-Histone H1 antibody (A06717) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-03
Question
I was wanting to use your anti-Histone H1 antibody for WB for mouse ovarian carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse ovarian carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
You can see on the product datasheet, A06717 anti-Histone H1 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse ovarian carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-14