Anti-HDAC2 Antibody (Monoclonal)
|Applications||ChIP, ChIP-qPCR, ChIP-seq, IF|
|Product Name||Anti-HDAC2 Antibody (Monoclonal)|
|Storage & Handling||At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.|
|Description||Mouse monoclonal antibody for human HDAC2 (Histone deacetylase 2).tested for ChIP, ChIP-qPCR, ChIP-seq, IF, IHC in Human.|
|Cite This Product||Anti-HDAC2 Antibody (Monoclonal) (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1000)|
|Contents/Buffer||Protein A purified monoclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.|
|Immunogen||This antibody is raised in mouse against human HDAC2 (Histone deacetylase 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.|
Assay Dilutions Overview
User need to optimize the dilution ratio for this antibody
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot. Boster recommends high sensitivity ChIP-seq Kit (CK1001 & CK1002) for Chromatin Immunoprecipitation.
Images And Assay Conditions
ChIP-qPCR data (% Recovery) with Anti-HDAC2 monoclonal antibody, optimized qPCR primers and sheared chromatin from 4 million human HeLa cells.
ChIP-seq data generated on Illumina HiSeq platform and analyzed with the BWA algorithm which aligns 51 bp tags to the mouse genome. Figures A and B show the enrichment along the complete sequence and a 2 Mb region of human chromosome 3. Two genomic region
WB result using whole cell extracts from HeLa Cells (25 μg) and 1:1,000 antibody dilution. Predicted band size: 55 KD. Observed band size: 55 KD.
Immunofluorescence images stained on HeLa cells: (Left) Cells stained with anti-HDAC2 monoclonal antibody (Catalog # CI1000) at 1/500 dilution. (Middle) Nuclei stained with DAPI. (Right) Merged images of two stains from the left and middle.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Prostaglandin E2 receptor EP2 subtype|
|Tissue Specificity||Placenta and lung.|
|Alternative Names||Prostaglandin E2 receptor EP2 subtype;PGE receptor EP2 subtype;PGE2 receptor EP2 subtype;Prostanoid EP2 receptor;PTGER2;|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Molecular Weight||39761 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.|
*You can search these to find other products in these research areas.
|Background||HDAC2 belongs to the family of histone deacetylases which catalyse the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression and HDAC2 activity is associated with gene repression. Histone deacetylation is established by the formation of large multiprotein complexes. Thereby, HDAC2 associates with many different proteins, including the mammalian zinc-finger transcription factor YY1. HDAC2 thus plays an important role in transcriptional regulation, cell cycle progression and developmental events.|
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