Anti-HDAC2 Antibody (Monoclonal)

SKU CI1000
Size 50μg/ 25μl
Reactivity Human
Clonality Monoclonal
Host Mouse
Applications ChIP, ChIP-qPCR, ChIP-seq, IF

Overview

Product Name Anti-HDAC2 Antibody (Monoclonal)
SKU/Catalog Number CI1000
Storage & Handling At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Size 50μg/ 25μl
Description Mouse monoclonal antibody for human HDAC2 (Histone deacetylase 2).tested for ChIP, ChIP-qPCR, ChIP-seq, IF, IHC in Human.
Cite This Product Anti-HDAC2 Antibody (Monoclonal) (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1000)
Host Mouse
Contents/Buffer Protein A purified monoclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Form Liquid
Immunogen This antibody is raised in mouse against human HDAC2 (Histone deacetylase 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
User need to optimize the dilution ratio for this antibody

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot. Boster recommends high sensitivity ChIP-seq Kit (CK1001 & CK1002) for Chromatin Immunoprecipitation.

Images And Assay Conditions

ChIP-qPCR data (% Recovery) with Anti-HDAC2 monoclonal antibody, optimized qPCR primers and sheared chromatin from 4 million human HeLa cells.

ChIP-seq data generated on Illumina HiSeq platform and analyzed with the BWA algorithm which aligns 51 bp tags to the mouse genome. Figures A and B show the enrichment along the complete sequence and a 2 Mb region of human chromosome 3. Two genomic region

WB result using whole cell extracts from HeLa Cells (25 μg) and 1:1,000 antibody dilution. Predicted band size: 55 KD. Observed band size: 55 KD.

Immunofluorescence images stained on HeLa cells: (Left) Cells stained with anti-HDAC2 monoclonal antibody (Catalog # CI1000) at 1/500 dilution. (Middle) Nuclei stained with DAPI. (Right) Merged images of two stains from the left and middle.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P43116
Gene Name PTGER2
Protein Name Prostaglandin E2 receptor EP2 subtype
Tissue Specificity Placenta and lung.
Alternative Names Prostaglandin E2 receptor EP2 subtype;PGE receptor EP2 subtype;PGE2 receptor EP2 subtype;Prostanoid EP2 receptor;PTGER2;
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Molecular Weight 39761 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Research Areas Human

*You can search these to find other products in these research areas.
Background HDAC2 belongs to the family of histone deacetylases which catalyse the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression and HDAC2 activity is associated with gene repression. Histone deacetylation is established by the formation of large multiprotein complexes. Thereby, HDAC2 associates with many different proteins, including the mammalian zinc-finger transcription factor YY1. HDAC2 thus plays an important role in transcriptional regulation, cell cycle progression and developmental events.

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In stock
Order Product
CI1000
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$380.00

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