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Product Info Summary
| SKU: | M05980-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HAPLN1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05980-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HAPLN1 Rabbit Monoclonal Antibody catalog # M05980-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HAPLN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05980-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BHA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HAPLN1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05980-1 is reactive to HAPLN1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
40166 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05980-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: Rat liver tissue, Mouse skeletal muscle - gastrocnemius tissue, Mouse heart tissue, Human glioblastoma tissue, human kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of HAPLN1 expression in (1) Caco2 cell lysate; (2) Mouse spleen lysate.
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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney, using HAPLN1 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For HAPLN1 (Source: Uniprot.org, NCBI)
Gene Name
HAPLN1
Full Name
Hyaluronan and proteoglycan link protein 1
Weight
40166 MW
Superfamily
HAPLN family
Alternative Names
Hyaluronan and proteoglycan link protein 1;Cartilage-linking protein 1;Cartilage-link protein;Proteoglycan link protein;HAPLN1;CRTL1; HAPLN1 CRT1, CRTL1 hyaluronan and proteoglycan link protein 1 hyaluronan and proteoglycan link protein 1|Cartilage link protein|cartilage-link protein|cartilage-linking protein 1|proteoglycan link protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HAPLN1, check out the HAPLN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HAPLN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HAPLN1 Rabbit Monoclonal Antibody (M05980-1)
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No publications found for M05980-1
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Customer Q&As
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1 Customer Q&As for Anti-HAPLN1 Rabbit Monoclonal Antibody
Question
We are currently using anti-HAPLN1 Rabbit Monoclonal antibody M05980-1 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
The anti-HAPLN1 Rabbit Monoclonal antibody (M05980-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-19
