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Product Info Summary
| SKU: | A06281-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Granzyme K/GZMK Antibody
View all Granzyme K Antibodies
SKU/Catalog Number
A06281-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Granzyme K/GZMK Antibody catalog # A06281-2. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Granzyme K/GZMK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06281-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Granzyme K/GZMK, which shares 71.4% and 73.9% amino acid (aa) sequence identity with mouse and rat GZMK, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06281-2 is reactive to GZMK in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
49655 MW
Background of Granzyme K
Granzyme K is a protein that in humans is encoded by the GZMK gene. This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06281-2 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Positive Control
IHC: human spleen tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Granzyme K/GZMK using anti-Granzyme K/GZMK antibody (A06281-2).
Granzyme K/GZMK was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Granzyme K/GZMK Antibody (A06281-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GZMK (Source: Uniprot.org, NCBI)
Gene Name
GZMK
Full Name
Granzyme K
Weight
49655 MW
Superfamily
peptidase S1 family
Alternative Names
ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL2; HUB GZMK TRYP2 granzyme K granzyme K|NK-Tryp-2|NK-tryptase-2|fragmentin-3|granzyme 3|granzyme K (granzyme 3; tryptase II)|granzyme K (serine protease, granzyme 3; tryptase II)|tryptase II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GZMK, check out the GZMK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GZMK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Granzyme K/GZMK Antibody (A06281-2)
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