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Product Info Summary
| SKU: | A30808 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-GPR142 Antibody
SKU/Catalog Number
A30808
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR142 Antibody (Catalog# A30808). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR142 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30808)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR142. Immunogen sequence location: 18264
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30808 is reactive to GPR142 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
51106 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30808 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells, using GPR142 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using GPR142 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For GPR142 (Source: Uniprot.org, NCBI)
Gene Name
GPR142
Full Name
Probable G-protein coupled receptor 142
Weight
51106 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
GPR142;PGR2;Probable G-protein coupled receptor 142;G-protein coupled receptor PGR2 Gpr142|PGR2|G protein-coupled receptor 142|probable G-protein coupled receptor 142|G protein-coupled receptor PGR2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPR142, check out the GPR142 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPR142: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPR142 Antibody (A30808)
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Customer Q&As
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5 Customer Q&As for Anti-GPR142 Antibody
Question
Is a blocking peptide available for product anti-GPR142 antibody (A30808)?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We do provide the blocking peptide for product anti-GPR142 antibody (A30808). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-12
Question
Can you help my question with product A30808, anti-GPR142 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30808 anti-GPR142 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-30
Question
We are currently using anti-GPR142 antibody A30808 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
The anti-GPR142 antibody (A30808) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-29
Question
I am interested in to test anti-GPR142 antibody A30808 on human ectocervix for research purposes, then I may be interested in using anti-GPR142 antibody A30808 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
The products we sell, including anti-GPR142 antibody A30808, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-13
Question
Does anti-GPR142 antibody A30808 work on canine WB with ectocervix?
A. Jha
Verified customer
Asked: 2016-04-01
Answer
Our lab technicians have not validated anti-GPR142 antibody A30808 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GPR142 antibody A30808 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine ectocervix in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-01
