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Product Info Summary
| SKU: | A03925 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-FLASH CASP8AP2 Antibody
SKU/Catalog Number
A03925
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FLASH CASP8AP2 Antibody (Catalog # A03925). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
FLASH antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FLASH CASP8AP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03925)
Host
Rabbit
Contents
FLASH Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid. The immunogen is located within the last 50 amino acids of FLASH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IFIT3 antibody is human specific. This antibody is predicted to not cross-react with other members of the IFIT protein family.
Reactive Species
A03925 is reactive to CASP8AP2 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
222658 MW
Background of FLASH
A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03925 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FLASH antibody can be used for detection of FLASH by Western blot at 0.5 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/mL.
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Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.
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Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CASP8AP2 (Source: Uniprot.org, NCBI)
Gene Name
CASP8AP2
Full Name
CASP8-associated protein 2
Weight
222658 MW
Alternative Names
CED-4, FLASH, RIP25, KIAA1315, CASP8-associated protein 2, FLICE-associated huge protein CASP8AP2 CED-4, FLASH, RIP25 caspase 8 associated protein 2 CASP8-associated protein 2|FLASH homolog RIP25|FLICE associated huge|FLICE-associated huge protein|human FLASH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP8AP2, check out the CASP8AP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP8AP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FLASH CASP8AP2 Antibody (A03925)
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