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Product Info Summary
| SKU: | A03336-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-ELL Antibody Picoband®
SKU/Catalog Number
A03336-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ELL Antibody Picoband® catalog # A03336-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ELL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03336-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ELL recombinant protein (Position: M1-S407).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03336-1 is reactive to ELL in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
60-80 kDa
Calculated molecular weight
68.265kDa
Background of ELL
RNA polymerase II elongation factor ELL is an enzyme that in humans is encoded by the ELL gene. Eleven-nineteen lysine-rich leukemia protein (ELL) was originally identified in a screen for genes that fuse to MLL (myeloid-lymphoid leukemia) in the t(11;19)(q23;p13.1) breakpoint. ELL is an elongation factor that increases the catalytic rate of RNA pol II by suppressing transient pausing of RNA pol II. ELL has been shown to be essential to development and important to cell growth and survival. Alternate names for ELL include RNA polymerase II elongation factor ELL, ELL1, and men.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03336-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human HEK293 whole cell, human HL-60 whole cell
ICC/IF: A431 cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ELL using anti-ELL antibody (A03336-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELL antigen affinity purified polyclonal antibody (Catalog # A03336-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELL at approximately 60-80 kDa. The expected band size for ELL is at 68 kDa.
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Figure 2. IF analysis of ELL using anti-ELL antibody (A03336-1).
ELL was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ELL Antibody (A03336-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of 293T cells using anti-ELL antibody (A03336-1).
Overlay histogram showing 293T cells stained with A03336-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ELL Antibody (A03336-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ELL (Source: Uniprot.org, NCBI)
Gene Name
ELL
Full Name
RNA polymerase II elongation factor ELL
Weight
68.265kDa
Superfamily
ELL/occludin family
Alternative Names
Glutathione S-transferase Mu 3; GST class-mu 3; GSTM3-3; hGSTM3-3; GSTM3; GST5; ELL C19orf171, MEN, PPP1R68, ELL elongation factor for RNA polymerase II RNA polymerase II elongation factor ELL|ELL gene (11-19 lysine-rich leukemia gene)|ELL/KMT2A fusion protein|KMT2A/ELL fusion protein|eleven-nineteen lysine-rich leukemia protein|elongation factor RNA polymerase II|protein phosphatase 1, regulatory subunit 68
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELL, check out the ELL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ELL Antibody Picoband® (A03336-1)
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