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Product Info Summary
| SKU: | A00410-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-DR5 TNFRSF10B Antibody
View all TRAILR2/TNFRSF10B Antibodies
SKU/Catalog Number
A00410-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DR5 TNFRSF10B Antibody (Catalog # A00410-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DR5 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DR5 TNFRSF10B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00410-2)
Host
Rabbit
Contents
DR5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DR5 antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human DR5 precursor. The immunogen is located within the last 50 amino acids of DR5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Antibody has no cross reaction to DR4.
Reactive Species
A00410-2 is reactive to TNFRSF10B in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
47878 MW
Background of TRAILR2/TNFRSF10B
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-κB.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00410-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IHC-P: 5 μg/mL; IF: 5-10 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: DR5 A00410-2, (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: DR5 A00410-2, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of DR5 in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling DR5 with A00410-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
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Immunohistochemistry Validation of DR5 in Mouse kidney tissue
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DR5 antibody (A00410-2) at 5μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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KD Validation of DR5 in MB231 Cells (Rahman et al., 2009)
Western blot analysis with anti-DR5 antibodies was performed for DR5 in MB231 cells transfected with control siRNA or DR5 siRNA. DR5 expression was disrupted after DR5 siRNA knockdown.
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KO Validation of DR5 in HCT116 Cells (Han et al., 2015)
Anti-cancer drug, Carfilzomib (CFZ), induced up-regulation of DR5 and the expression of DR5 was not detected in DR5-KO HCT 116 cell line with anti-DR5 antibodies (A00410-2).
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Immunohistochemistry Validation of BIM in Human Colon Tumors (Devetzi et al., 2016)
Protein analysis for DR5 by immunohistochemistry with anti-DR5 antibodies in human colon tumors. Strong immunoreactivity is shown for DR5 in T167 patient with colorectal cancer.
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Regulated Expression Validation of DR5 in Thyroid Epithelial Cells (Bretz et al., 2002)
Immunostaining with anti-DR5 antibodies shows high levels of DR5 expression in untreated cells and cells treated with each of the three cytokines alone or TNFalpha combined with IL-1b. In contrast, treatment with both IFNg and TNFalpha or all three cytokines greatly reduces DR5 staining. The reduction in staining appears most significant in cytoplasmic regions while some staining is maintained in or around the nucleus.
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Immunofluorescence Validation of DR5 in Rat Brain Tissue with Tumors (Candolfi et al., 2009)
Rats were implanted in the striatum with CNS-1 tumors and 9 days later brains were processed for immunocytochemistry. Confocal microphotographs show detection of therapeutic targets (green) using specific antibodies against the death receptors TNFR1 and TRAILR2. Tumor cells were labeled with anti-vimentin antibodies (red), neurons were stained with anti-NeuN (red) and astrocytes with anti-GFAP antibodies (red). Nuclei were stained with DAPI (blue). T: tumor area. N: necrotic patch.
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF10B (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF10B
Full Name
Tumor necrosis factor receptor superfamily member 10B
Weight
47878 MW
Alternative Names
DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5, DR5, UNQ160/PRO186, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TRAIL receptor 2 TNFRSF10B CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 TNF receptor superfamily member 10b tumor necrosis factor receptor superfamily member 10B|Fas-like protein|TNF-related apoptosis-inducing ligand receptor 2|apoptosis inducing protein TRICK2A/2B|apoptosis inducing receptor TRAIL-R2|cytotoxic TRAIL receptor-2|death domain containing receptor for TRAIL/Apo-2L|death receptor 5|p53-regulated DNA damage-inducible cell death receptor(killer)|tumor necrosis factor receptor superfamily, member 10b|tumor necrosis factor receptor-like protein ZTNFR9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF10B, check out the TNFRSF10B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF10B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DR5 TNFRSF10B Antibody (A00410-2)
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