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Product Info Summary
| SKU: | M00745-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Cyclin B1 CCNB1 Monoclonal Antibody
SKU/Catalog Number
M00745-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cyclin B1 CCNB1 Monoclonal Antibody catalog # M00745-1. Tested in ELISA, Flow Cytometry, IF, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin B1 CCNB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00745-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5G6
Isotype
IgG
Immunogen
Purified recombinant fragment of human Cyclin B1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00745-1 is reactive to CCNB1 in Human, Rat
Reconstitution
Calculated molecular weight
48337 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00745-1 is guaranteed for ELISA, Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 3. Flow Cytometry validation of CCNB1 using Anti-Cyclin B1 CCNB1 Monoclonal Antibody (M00745-1).
Flow cytometric (FCM) analysis of HeLa cells using Cyclin B1 Monoclonal Antibody (green) and negative control (purple).
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Figure 2. Immunofluorescent staining data of CCNB1 using Anti-Cyclin B1 CCNB1 Monoclonal Antibody (M00745-1).
Immunofluorescence (IF) analysis of HeLa cells using Cyclin B1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 1. Western blotting validation for Anti-Cyclin B1 CCNB1 Monoclonal Antibody M00745-1
Western Blot (WB) analysis using Cyclin B1 Monoclonal Antibody against HeLa (1) and PC-12 (2) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CCNB1 (Source: Uniprot.org, NCBI)
Gene Name
CCNB1
Full Name
G2/mitotic-specific cyclin-B1
Weight
48337 MW
Superfamily
cyclin family
Alternative Names
G2/mitotic-specific cyclin-B1;CCNB1;CCNB; CCNB1 CCNB cyclin B1 G2/mitotic-specific cyclin-B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNB1, check out the CCNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin B1 CCNB1 Monoclonal Antibody (M00745-1)
Hello CJ!
M00745-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang J,Wang L,Chen S,Peng H,Xiao L,E Du,Liu Y,Lin D,Wang Y,Xu Y,Yang K.PKMYT1 is associated with prostate cancer malignancy and may serve as a therapeutic target.Gene.2020 Jun 20;744:144608.doi:10.1016/j. gene.2020.144608.Epub 2020 Mar 29.PMID:32234541.
Species: Human
M00745-1 usage in article: APP:WB, SAMPLE:LNCAP CELL,C4-2 CELL,PC-3 CELL AND DU 145 CELL, DILUTION:1:200
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
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