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Product Info Summary
| SKU: | A02747-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-CXADR Antibody Picoband®
SKU/Catalog Number
A02747-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXADR Antibody Picoband® catalog # A02747-1. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CXADR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02747-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human CXADR recombinant protein (Position: E35-V365). Human CXADR shares 91.2% and 91.5% amino acid (aa) sequence identity with mouse and rat CXADR, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02747-1 is reactive to CXADR in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
40.03kDa
Background of CXADR
CXADR(Coxsackie virus and adenovirus receptor) is a protein that in humans is encoded by the CXADR gene, also known as CAR,CVB3-binding protein, Coxsackievirus B-adenovirus receptor. The CAR cDNA encodes a predicted 365-amino acid polypeptide that contains a single transmembrane domain and is a member of the immunoglobulin superfamily. By Northern blot analysis, they detected highest expression of 1.4-kb and 6-kb CXADR transcripts in pancreas, brain, heart, small intestine, testis, and prostate, lower expression in liver and lung, and no expression in kidney, placenta, peripheral blood leukocytes, thymus, and spleen. In comparison, mouse Cxadr showed highest expression in liver, and lower levels in kidney, heart, lung, and brain. The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins , such as: fibronectin, agrin, laminin-1 and tenascin-R.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02747-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human A431 whole cell, human HepG2 whole cell, monkey COS-7 whole cell, rat heart tissue, mouse heart tissue
ICC/IF: U2OS cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CXADR using anti-CXADR antibody (A02747-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXADR antigen affinity purified polyclonal antibody (Catalog # A02747-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXADR at approximately 45 kDa. The expected band size for CXADR is at 40 kDa.
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Figure 2. IF analysis of CXADR using anti-CXADR antibody (A02747-1).
CXADR was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CXADR Antibody (A02747-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HepG2 cells using anti-CXADR antibody (A02747-1).
Overlay histogram showing HepG2 cells stained with A02747-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CXADR Antibody (A02747-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CXADR (Source: Uniprot.org, NCBI)
Gene Name
CXADR
Full Name
Coxsackievirus and adenovirus receptor
Weight
40.03kDa
Alternative Names
CAR10Coxsackievirus B-adenovirus receptor; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; coxsackievirus and adenovirus receptor; CVB3 binding protein; CVB3 BP; CVB3-binding protein; CXADR; HCAR; HCVADR CXADR CAR, CAR4/6, HCAR CXADR Ig-like cell adhesion molecule coxsackievirus and adenovirus receptor|46 kD coxsackievirus and adenovirus receptor (CAR) protein|CVB3-binding protein|HCVADR|coxsackie virus and adenovirus receptor|coxsackievirus B-adenovirus receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXADR, check out the CXADR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXADR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXADR Antibody Picoband® (A02747-1)
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