Click image to see more details
Product Info Summary
| SKU: | A00788-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Collagen III COL3A1 Antibody
View all Collagen III alpha 1/COL3A1 Antibodies
SKU/Catalog Number
A00788-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Collagen III COL3A1 Antibody (Catalog # A00788-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen III COL3A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00788-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human collagen III.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00788-1 is reactive to COL3A1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
138564 MW
Background of Collagen III alpha 1/COL3A1
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Arja Lamberg, J. Biol. Chem., May 1996; 271: 11988.
GW Conrad, J. Cell Biol., Mar 1980; 84: 501.
DR Keene, J. Cell Biol., Nov 1987; 105: 2393.
HM Wang, J. Histochem. Cytochem., Nov 1980; 28: 1215.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00788-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using Collagen III antibody A00788-1.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using Collagen III antibody A00788-1.
Protein Target Info & Infographic
Gene/Protein Information For COL3A1 (Source: Uniprot.org, NCBI)
Gene Name
COL3A1
Full Name
Collagen alpha-1(III) chain
Weight
138564 MW
Superfamily
fibrillar collagen family
Alternative Names
Collagen alpha-1 (III) chain;COL3A1; COL3A1 EDS4A, EDSVASC, PMGEDSV collagen type III alpha 1 chain collagen alpha-1(III) chain|Ehlers-Danlos syndrome type IV, autosomal dominant|alpha-1 type III collagen|alpha1 (III) collagen|collagen, fetal|collagen, type III, alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL3A1, check out the COL3A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL3A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen III COL3A1 Antibody (A00788-1)
Hello CJ!
A00788-1 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Therapeutic effects of continuous infusion of brain natriuretic peptides on postmyocardial infarction ventricular remodelling in rats
Continuing Treatment with Salvia miltiorrhiza Injection Attenuates Myocardial Fibrosis in Chronic Iron-Overloaded Mice
Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.
Effects of pentoxifylline on the hepatic content of TGF-?1 and collagen in Schistosomiasis japonica mice with liver fibrosis
Attenuation of portal hypertension by natural taurine in rats with liver cirrhosis
Ge Ws, Wang Yj, Wu Jx, Fan Jg, Chen Yw, Zhu L. Mol Med Rep. 2014 Jun;9(6):2145-51. Doi: 10.3892/Mmr.2014.2099. Epub 2014 Apr 1. ??-Catenin Is Overexpressed In Hepatic Fibrosis And Blockage Of Wnt/??-Catenin Signaling Inhibits Hepatic Stellate Cell...
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...
Xu Y, Zhang Ja, Xu Y, Guo Sl, Wang S, Wu D, Wang Y, Luo D, Zhou Br. Stem Cells Dev. 2015 May 1;24(9):1096-111. Doi: 10.1089/Scd.2014.0321. Epub 2015 Jan 21. Antiphotoaging Effect Of Conditioned Medium Of Dedifferentiated Adipocytes On Skin In Vivo...
Ge Ws, Wu Jx, Fan Jg, Wang Yj, Chen Yw. World J Gastroenterol. 2011 Sep 28;17(36):4090-8. Doi: 10.3748/Wjg.V17.I36.4090. Inhibition Of High-Mobility Group Box 1 Expression By Sirna In Rat Hepatic Stellate Cells.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Collagen III COL3A1 Antibody
Question
See attached the WB image, lot number and protocol we used for skin fibroblast using anti-Collagen III antibody A00788-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-07
Question
We are currently using anti-Collagen III antibody A00788-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The anti-Collagen III antibody (A00788-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-25
Question
I see that the anti-Collagen III antibody A00788-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-16
Question
I was wanting to use your anti-Collagen III antibody for IF for mouse skin fibroblast on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse skin fibroblast identification?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It shows on the product datasheet, A00788-1 anti-Collagen III antibody has been validated for IF, IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse skin fibroblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
My colleagues were content with the WB result of your anti-Collagen III antibody. However we have observed positive staining in liver extracellular space using this antibody. Is that expected? Could you tell me where is COL3A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
Based on literature, liver does express COL3A1. Generally COL3A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886
Boster Scientific Support
Answered: 2019-09-04
Question
Can you help my question with product A00788-1, anti-Collagen III antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00788-1 anti-Collagen III antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-19
Question
Is a blocking peptide available for product anti-Collagen III antibody (A00788-1)?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do provide the blocking peptide for product anti-Collagen III antibody (A00788-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-18
Question
We ordered your anti-Collagen III antibody for IF on placenta last year. I am using mouse, and We are going to use the antibody for IHC next. We want examining placenta as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
I have checked the website and datasheets of our anti-Collagen III antibody and it seems that A00788-1 has been tested on mouse in both IF and IHC. Thus A00788-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-23
Question
We need using your anti-Collagen III antibody for skin development studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
We appreciate your inquiry. This A00788-1 anti-Collagen III antibody is validated on human pancreas tissue, hela cells. It is guaranteed to work for IF, IHC in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-02
Question
Would A00788-1 anti-Collagen III antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Parker
Verified customer
Asked: 2018-09-18
Answer
It shows on the product datasheet, A00788-1 anti-Collagen III antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-18
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin fibroblast using anti-Collagen III antibody A00788-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-11
Question
Is there a BSA free version of anti-Collagen III antibody A00788-1 available?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen III antibody A00788-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-13
Question
We have seen staining in human placenta. What should we do? Is anti-Collagen III antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
From literature placenta does express COL3A1. From Uniprot.org, COL3A1 is expressed in uterine cervix, skin fibroblast, liver, placenta, colon carcinoma, among other tissues. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886
Boster Scientific Support
Answered: 2017-08-31
Question
I am interested in to test anti-Collagen III antibody A00788-1 on mouse skin fibroblast for research purposes, then I may be interested in using anti-Collagen III antibody A00788-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Thomas
Verified customer
Asked: 2017-07-24
Answer
The products we sell, including anti-Collagen III antibody A00788-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-24
Question
Is this A00788-1 anti-Collagen III antibody reactive to the isotypes of COL3A1?
N. Gonzalez
Verified customer
Asked: 2014-09-17
Answer
The immunogen of A00788-1 anti-Collagen III antibody is Synthesized peptide derived from internal of human collagen III. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-09-17
Question
Would anti-Collagen III antibody A00788-1 work for IF with skin fibroblast?
P. Johnson
Verified customer
Asked: 2014-08-22
Answer
According to the expression profile of skin fibroblast, COL3A1 is highly expressed in skin fibroblast. So, it is likely that anti-Collagen III antibody A00788-1 will work for IF with skin fibroblast.
Boster Scientific Support
Answered: 2014-08-22
