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Product Info Summary
| SKU: | A01060S280 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Chk1 (phospho-S280) CHEK1 Antibody
SKU/Catalog Number
A01060S280
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Chk1 (phospho-S280) CHEK1 Antibody catalog # A01060S280. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chk1 (phospho-S280) CHEK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01060S280)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01060S280 is reactive to CHEK1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
54434 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01060S280 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-Chk1 (phospho-S280) CHEK1 Antibody A01060S280
Western blot (WB) analysis of Chk1 (phospho-S280) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate(40ug)
Lane3:HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug)
Lane4:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug)
Lane5:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CHEK1 (Source: Uniprot.org, NCBI)
Gene Name
CHEK1
Full Name
Serine/threonine-protein kinase Chk1
Weight
54434 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase Chk1;2.7.11.1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1;CHEK1;CHK1; CHEK1 CHK1 checkpoint kinase 1 serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1|Chk1-S|cell cycle checkpoint kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHEK1, check out the CHEK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHEK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chk1 (phospho-S280) CHEK1 Antibody (A01060S280)
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No publications found for A01060S280
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