Anti-CD4 Antibody (Monoclonal, CA-4)

SKU MA1016
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number CA-4
Host Mouse
Ig Isotype Mouse IgG1
Applications Flow Cytometry, IHC, ICC

Overview

Product Name Anti-CD4 Antibody (Monoclonal, CA-4)
SKU/Catalog Number MA1016
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for CD4, CD4 molecule (CD4) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.
Cite This Product Anti-CD4 Antibody (Monoclonal, CA-4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1016)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Images And Assay Conditions

Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (MA1016).
Overlay histogram showing human PBMC cells stained with MA1016 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P05540
Gene Name Cd4
Protein Name T-cell surface glycoprotein CD4
Alternative Names T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;W3/25 antigen;CD4;Cd4;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 51438 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
Research Areas Human

*You can search these to find other products in these research areas.
Background CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

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In stock
Order Product
MA1016
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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Publications

Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.
Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X. Int J Nanomedicine. 2015 Jan 6;10:347-55. Doi: 10.2147/Ijn.S71245. Ecollection 2015. Treating Cutaneous Squamous Cell Carcinoma Using 5-Aminolevulinic Acid Pol...
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W. Nephron Exp Nephrol. 2014;128(1-2):46-56. Doi: 10.1159/000366446. Epub 2014 Nov 5. Increased Macrophage Activation Inhibited By Tacrolimus In The Kidney Of Diabetic Rats.
Chen X, Wan J, Liu J, Xie W, Diao X, Xu J, Zhu B, Chen Z. Lung Cancer. 2010 Sep;69(3):348-54. Doi: 10.1016/J.Lungcan.2009.11.013. Increased Il-17-Producing Cells Correlate With Poor Survival And Lymphangiogenesis In Nsclc Patients.
Ouyang W, An T, Guo D, Wu S, Tien P. Aids Res Hum Retroviruses. 2013 Mar;29(3):613-20. Doi: 10.1089/Aid.2012.0059. Epub 2013 Jan 11. The Potent Human Immunodeficiency Virus Type 1 (Hiv-1) Entry Inhibitor Hr212 Blocks Formation Of The Envelope Glyc...

Customer Q&As

  • Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applications
    A: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
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