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Product Info Summary
| SKU: | M00048-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Caspase-1 + p10 + p12 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00048-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Caspase-1 + p10 + p12 Rabbit Monoclonal Antibody catalog # M00048-2. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-1 + p10 + p12 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00048-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
26C50
Isotype
IgG
Immunogen
A synthesized peptide derived from Caspase-1 + p10 + p12
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00048-2 is reactive to CASP1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
45159 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00048-2 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Caspase-1 using anti-Caspase-1 antibody (M00048-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: mouse EL-4 whole cell lysates,
Lane 6: mouse J774A.1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-1 antigen affinity purified monoclonal antibody (Catalog # M00048-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-1 at approximately 45 kDa. The expected band size for Caspase-1 is at 45 kDa.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For CASP1 (Source: Uniprot.org, NCBI)
Gene Name
CASP1
Full Name
Caspase-1
Weight
45159 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-1;CASP-1;3.4.22.36;Interleukin-1 beta convertase;IL-1BC;Interleukin-1 beta-converting enzyme;ICE;IL-1 beta-converting enzyme;p45;Caspase-1 subunit p20;Caspase-1 subunit p10;CASP1;IL1BC, IL1BCE; CASP1 ICE, IL1BC, P45 caspase 1 caspase-1|CASP1 nirs variant 1|IL-1 beta-converting enzyme|IL1B-convertase|caspase 1, apoptosis-related cysteine peptidase|caspase-1 isoform alpha|interleukin 1, beta, convertase|interleukin 1-B converting enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP1, check out the CASP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-1 + p10 + p12 Rabbit Monoclonal Antibody (M00048-2)
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