Product Info Summary
SKU: | A00246-5 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Aurora A/Aurka Antibody Picoband®
SKU/Catalog Number
A00246-5
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aurora A/Aurka Antibody Picoband® catalog # A00246-5. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Aurora A/Aurka Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00246-5)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Aurora A/Aurka recombinant protein (Position: M1-S395).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00246-5 is reactive to Aurka in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
106600 MW
Background of Aurora A
AURKA(aurora kinase A), also called ARK1, AurA, AIK , AURORA2 ,BTAK, PPP1R47, STK7, STK15,STK6, is a mitotic centrosomal protein kinase. The main role of AURKA in tumor development is in controlling chromosome segregation during mitosis. Aurora A is a member of a family of mitotic serine/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of AURKA occurs during the G2/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Activation of AURKA was independently sufficient to induce rapid ciliary resorption, and AURKA acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of AURKA and HDAC6 reduced regulated disassembly of cilia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00246-5 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, Mouse
Positive Control
WB: rat testis tissue, mouse testis tissue, mouse thymus tissue, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Aurora A/Aurka using anti-Aurora A/Aurka antibody (A00246-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates,
Lane 3: mouse thymus tissue lysates,
Lane 4: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora A/Aurka antigen affinity purified polyclonal antibody (Catalog # A00246-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora A/Aurka at approximately 48 kDa. The expected band size for Aurora A/Aurka is at 46 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Aurka (Source: Uniprot.org, NCBI)
Gene Name
Aurka
Full Name
Aurora kinase A
Weight
106600 MW
Superfamily
protein kinase superfamily
Alternative Names
Probable ATP-dependent RNA helicase DDX58;3.6.4.13;DEAD box protein 58;RIG-I-like receptor 1;RLR-1;Retinoic acid-inducible gene 1 protein;RIG-1;Retinoic acid-inducible gene I protein;RIG-I;DDX58; AURKA AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7 aurora kinase A aurora kinase A|aurora 2|aurora/IPL1-like kinase|aurora/IPL1-related kinase 1|breast tumor-amplified kinase|protein phosphatase 1, regulatory subunit 47|serine/threonine protein kinase 15|serine/threonine-protein kinase 6|serine/threonine-protein kinase aurora-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Aurka, check out the Aurka Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Aurka: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aurora A/Aurka Antibody Picoband® (A00246-5)
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