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Product Info Summary
| SKU: | A06828-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Aprataxin and PNK-like factor APLF Antibody
SKU/Catalog Number
A06828-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Aprataxin and PNK-like factor APLF Antibody catalog # A06828-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aprataxin and PNK-like factor APLF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06828-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human APLF. AA range:82-131
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06828-1 is reactive to APLF in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
56956 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06828-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HT-29 cells using APLF Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For APLF (Source: Uniprot.org, NCBI)
Gene Name
APLF
Full Name
Aprataxin and PNK-like factor
Weight
56956 MW
Superfamily
APLF family
Alternative Names
Aprataxin and PNK-like factor;4.2.99.18;Apurinic-apyrimidinic endonuclease APLF;PNK and APTX-like FHA domain-containing protein;XRCC1-interacting protein 1;APLF;C2orf13, PALF, XIP1;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APLF, check out the APLF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APLF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aprataxin and PNK-like factor APLF Antibody (A06828-1)
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Customer Q&As
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6 Customer Q&As for Anti-Aprataxin and PNK-like factor APLF Antibody
Question
I see that the anti-APLF antibody A06828-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-21
Question
My lab would like to test anti-APLF antibody A06828-1 on mouse corpus callosum for research purposes, then I may be interested in using anti-APLF antibody A06828-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Jackson
Verified customer
Asked: 2019-01-07
Answer
The products we sell, including anti-APLF antibody A06828-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-07
Question
I have a question about product A06828-1, anti-APLF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06828-1 anti-APLF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-14
Question
Is a blocking peptide available for product anti-APLF antibody (A06828-1)?
Verified Customer
Verified customer
Asked: 2018-05-28
Answer
We do provide the blocking peptide for product anti-APLF antibody (A06828-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-28
Question
I was wanting to use your anti-APLF antibody for WB for mouse corpus callosum on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse corpus callosum identification?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
It shows on the product datasheet, A06828-1 anti-APLF antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse corpus callosum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-21
Question
We are currently using anti-APLF antibody A06828-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
J. Rodriguez
Verified customer
Asked: 2016-09-05
Answer
The anti-APLF antibody (A06828-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-05
