Product Info Summary
SKU: | PB9738 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-AMPK beta 1/PRKAB1 Antibody Picoband®
View all AMPK beta 1 Antibodies
SKU/Catalog Number
PB9738
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AMPK beta 1/PRKAB1 Antibody Picoband® catalog # PB9738. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK beta 1/PRKAB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9738)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 1, different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9738 is reactive to PRKAB1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
30382 MW
Background of AMPK beta 1
5'-AMP-activated protein kinase subunit beta-1 is an enzyme that in humans is encoded by the PRKAB1 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9738 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: JURKAT Whole Cell, PANC Whole Cell, K562 Whole Cell
ICC/IF: A431 cell
Validation Images & Assay Conditions
![pb9738 1 WB anti ampk beta 1 picoband antibody pb9738 1 WB anti ampk beta 1 picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb9738-1-WB-anti-ampk-beta-1-picoband-antibody.jpg)
Click image to see more details
Figure 1. Western blot analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: JURKAT Whole Cell Lysate,
Lane 2: PANC Whole Cell Lysate,
Lane 3: K562 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK beta 1 antigen affinity purified polyclonal antibody (Catalog # PB9738) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK beta 1 at approximately 38 kDa. The expected band size for AMPK beta 1 is at 30 kDa.
![pb9738 2 pb9738 2](https://www.bosterbio.com/media/catalog/product/p/b/pb9738-2.jpg)
Click image to see more details
Figure 2. IF analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738).
AMPK beta 1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AMPK beta 1 Antibody (PB9738) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PRKAB1 (Source: Uniprot.org, NCBI)
Gene Name
PRKAB1
Full Name
5'-AMP-activated protein kinase subunit beta-1
Weight
30382 MW
Superfamily
5'-AMP-activated protein kinase beta subunit family
Alternative Names
5'-AMP-activated protein kinase subunit beta-1;AMPK subunit beta-1;AMPKb;PRKAB1;AMPK; PRKAB1 AMPK, HAMPKb protein kinase AMP-activated non-catalytic subunit beta 1 5-AMP-activated protein kinase subunit beta-1|5-AMP-activated protein kinase beta-1 subunit|AMP-activated protein kinase beta subunit|AMPK beta -1 chain|AMPK beta 1|AMPK subunit beta-1|AMPKb|protein kinase, AMP-activated, beta 1 non-catalytic subunit|protein kinase, AMP-activated, noncatalytic, beta-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAB1, check out the PRKAB1 Infographic
![PRKAB1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMPK beta 1/PRKAB1 Antibody Picoband® (PB9738)
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Customer Q&As
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3 Customer Q&As for Anti-AMPK beta 1/PRKAB1 Antibody Picoband®
Question
We are currently using anti-AMPK beta 1/PRKAB1 antibody PB9738 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The anti-AMPK beta 1/PRKAB1 antibody (PB9738) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-05
Question
I was wanting to use your anti-AMPK beta 1/PRKAB1 antibody for WB for human lung muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung muscle identification?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
It shows on the product datasheet, PB9738 anti-AMPK beta 1/PRKAB1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-05
Question
Will anti-AMPK beta 1/PRKAB1 antibody PB9738 work for WB with lung muscle?
C. Carter
Verified customer
Asked: 2015-12-03
Answer
According to the expression profile of lung muscle, PRKAB1 is highly expressed in lung muscle. So, it is likely that anti-AMPK beta 1/PRKAB1 antibody PB9738 will work for WB with lung muscle.
Boster Scientific Support
Answered: 2015-12-03