Anti-AMPK beta 1/PRKAB1 Antibody Picoband®

Product Name

Anti-AMPK beta 1/PRKAB1 Antibody Picoband®

View all AMPK beta 1 Antibodies

SKU/Catalog Number

PB9738

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AMPK beta 1/PRKAB1 Antibody Picoband® catalog # PB9738. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AMPK beta 1/PRKAB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9738)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 1, different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9738 is reactive to PRKAB1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

38 kDa

Calculated molecular weight

30382 MW

Background of AMPK beta 1

5'-AMP-activated protein kinase subunit beta-1 is an enzyme that in humans is encoded by the PRKAB1 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9738 is guaranteed for IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Positive Control

WB: JURKAT Whole Cell, PANC Whole Cell, K562 Whole Cell
ICC/IF: A431 cell

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: JURKAT Whole Cell Lysate,
Lane 2: PANC Whole Cell Lysate,
Lane 3: K562 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK beta 1 antigen affinity purified polyclonal antibody (Catalog # PB9738) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK beta 1 at approximately 38 kDa. The expected band size for AMPK beta 1 is at 30 kDa.

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Figure 2. IF analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738).
AMPK beta 1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AMPK beta 1 Antibody (PB9738) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PRKAB1 (Source: Uniprot.org, NCBI)

Alternative Names

5'-AMP-activated protein kinase subunit beta-1;AMPK subunit beta-1;AMPKb;PRKAB1;AMPK; PRKAB1 AMPK, HAMPKb protein kinase AMP-activated non-catalytic subunit beta 1 5-AMP-activated protein kinase subunit beta-1|5-AMP-activated protein kinase beta-1 subunit|AMP-activated protein kinase beta subunit|AMPK beta -1 chain|AMPK beta 1|AMPK subunit beta-1|AMPKb|protein kinase, AMP-activated, beta 1 non-catalytic subunit|protein kinase, AMP-activated, noncatalytic, beta-1

For more info on PRKAB1, check out the PRKAB1 Infographic

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In this infographic, you will see the following information for PRKAB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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